The principal topical antimicrobial in use today for decolonization (mupirocin) was approved by the US FDA nearly two decades ago for ‘the eradication of nasal colonization with MRSA’ and completed its ‘microbiology review’ in July 1997 for the treatment of small wounds. aureus or MRSA (Methicillin-resistant Staphylococcus aureus) is now a known independent risk factor linked to increasing incidence and severity of infection after surgery. Nasal decolonization of Staphylococcus aureus has been accepted as an established prophylactic therapy to prevent post-operative infection, as colonization with S. through decontamination/ prophylaxis pre-surgery) is a more biologically plausible approach to reducing S. aureus load around highrisk procedures (e.g. aureus eradication as a concept, and suggest that reducing S.
Miller suggested that their findings " question the validity of S. aureus in the next swab, but also increased the likelihood of later acquisition ". When these data54 555657 are examined in light of (a) our previously published photo-biologic efflux attenuation data, (b) previously published photobiologic potentiation of erythromycin topical against MRSA in the human nares, and (c) the newly presented in vitro data showing apparent inhibition of biofilm formation in MRSA, after 870 nm/930 nm sub-lethal irradiation with a mupirocin challenge, there is a likelihood that date (571 patients cultured every 60 days and followed for median two years) reported that those patients receiving anti-staphylococcal antibiotics " significantly increased the likelihood of losing S. These data suggest that inhibition of PMF-dependent trasporters might decrease biofilm formation in P. The PMF inhibitor CCCP effect was correlated with the expression of MexAB-OprM efflux system and found to compromise biofilm formation in P. Biofilms of the other two isolates and control strain PA140 exhibited significantly lower absorbance (P ranging from < 001 to < 0.05) with either 12.5 or 25 micromol l(-1) of CCCP. Two isolates subexpressed mexB gene and only 25 micromol l(-1) of CCCP affected biofilm formation.
Mean values of optical density were analysed with one-way analysis of variance and t-test. Expression of gene mexB was examined and biofilm formation after incubation with 0, 125 and 25 micromol l(-1) of CCCP was investigated. A MexAB-oprM overproducing strain was used as control. We evaluated the effect of the PMF inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development.įour epidemiologically unrelated P. Proton motive force (PMF) inhibition enhances the intracellular accumulation of autoinducers possibly interfering with biofilm formation.
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